kikogarcia

kikogarcia hat ein neuen Blog hinzugefügt Health
2025-06-11 09:22:31 - Übersetzen -

Introduction to Amplicon Sequence Variants
In recent years, amplicon sequencing technology has emerged as a critical tool for studying microbiomes, oncology, and genetics. By performing high-throughput sequencing of specific genetic regions, researchers can unravel the structure, functions, and dynamic changes of microbial communities from complex samples. In amplicon sequencing data analysis, the amplicon sequence variant...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-06-11 09:18:22 - Übersetzen -

Definition & Principle, Workflow, and Applications of Dual RNA Sequencing
What is dual RNA sequencing? Understanding how a pathogen induces disease requires knowledge of which genes are expressed and how they are regulated during infection. RNA sequencing has been routinely used to analyze the gene expression of microbial pathogens. Apart from the standard mRNA sequencing, new methods are surging based on RNA-seq, mapping the post-transcriptional networks controlled...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-06-11 09:07:12 - Übersetzen -

Microarray Data Analysis Pipeline
What is Microarray Microarray is a powerful experimental platform that can simultaneously study the expression of thousands of genes under different experimental conditions. The common microarray techniques include SNP microarray, CGH microarray, gene expression using DNA microarrays, DNA methylation microarray, etc. The overall goal of...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-04-09 01:40:26 - Übersetzen -

The Power of Iso-Seq in Gene Discovery and Annotation
Gene discovery and annotation serve as essential cornerstones in our ongoing endeavor to decipher the intricate mysteries of biological processes, disease mechanisms, and evolutionary connections. They provide the foundational knowledge needed to advance our understanding of life at its most fundamental level. Over the years, conventional RNA-Seq has played a pivotal role in gene...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-04-09 01:37:00 - Übersetzen -

Dual RNA Sequencing: Definition & Principle, Workflow, and Applications
What is dual RNA sequencing? Understanding how pathogen induces disease requires knowledge of which genes are expressed and how they are regulated during infection. RNA sequencing has been routinely used to analyze the gene expression of microbial pathogens. Apart from the standard mRNA sequencing, new methods are surging based on RNA-seq, mapping the post-transcriptional networks controlled...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-02-05 07:32:20 - Übersetzen -

Summary of Common Databases for Next Generation Sequencing
The Role of the NGS Sequencing Database In light of the accelerated advancements in technology, especially the meteoric evolution within the domain of bioinformatics, the significance of Next-Generation Sequencing (NGS) technologies as an essential instrument in life science research has markedly increased. Distinguished by its high throughput, efficiency, and unerring accuracy, NGS...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2025-02-05 07:23:01 - Übersetzen -

Bioinformatics Workflow of Whole Exome Sequencing
Overview of WES The advent of next-generation sequencing (NGS) has greatly accelerated genomics research, which produces millions to billions of sequence reads at a high speed. Currently, available NGS platforms include Illumina, Ion Torrent/Life Technologies, 454/Roche, Pacific Bioscience, Nanopore, and GenapSys. They can produce reads of 100-10,000 bp in length, enabling sufficient coverage...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-12-20 03:19:21 - Übersetzen -

Principles，Analysis, Advantages and Implications of Hi-C technology
What is Hi-C technology? Hi-C is a high-throughput genomic and epigenomic technique designed to capture the three-dimensional (3D) conformation of chromatin within the nucleus. Chromatin, which packages long DNA strands in a confined nuclear volume, plays a crucial role in biological functions at both the gene and global nuclear levels. Understanding chromatin organization is...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-12-20 03:15:50 - Übersetzen -

A Tool for Analyzing Epigenetics: ATAC-seq
Chromatin Dynamics and Gene Regulation in Eukaryotic Cells The total length of DNA within human cells approximates two meters and necessitates complex folding processes to be accommodated within the nucleus. During this dynamic folding process, chromatin exhibits heterogeneous structural states, manifesting as either open or closed configurations. The open state of chromatin is crucial for the...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-10-28 03:00:00 - Übersetzen -

RNA-Seq Library Construction for Illumina Platform
What is Library in Sequencing? The construction of DNA and RNA libraries, spanning from the initial fragmentation to PCR amplification, is a pivotal process in molecular biology. A gene library serves as a repository of receptor bacteria harboring DNA fragments from a specific organism. These libraries can be broadly classified into genomic libraries, encompassing all DNA fragments, and cDNA...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-10-28 02:57:42 - Übersetzen -

A Beginner's Guide to Single-cell RNA Sequencing
The Type of Sequencing It's important to determine the type of sequencing you need before starting an RNA sequencing experiment. Bulk cell RNA sequencing (bulk RNA-seq) measures the average gene expression across a tissue sample, while single-cell RNA sequencing (scRNA-seq) measures gene expression at the individual cell level. Factors such as time, cost, workload, and...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-07-04 08:25:47 - Übersetzen -

Long-read Sequencing for DNA 5-methylcytosine (5mC) Analysis
Overview DNA methylation is a fundamental epigenetic modification process that regulates gene expression and cellular responses to stimuli. Several different types of methylation, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and n6 -methyladenosine (6mA), depending on the position of the atom in adenine or cytosine that is being modified, are among the most widely...

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kikogarcia hat ein neuen Blog hinzugefügt Health
2024-07-04 08:23:25 - Übersetzen -

Nanopore Adaptive Sampling Revolutionizes Target Enrichment
Overview of Adaptive Sampling In a groundbreaking advancement for genomics, the concept of Nanopore Adaptive Sampling has emerged as a transformative strategy, pushing the boundaries of target enrichment. This novel approach introduces a paradigm shift by seamlessly integrating target enrichment or depletion directly into the sequencing process. This eliminates the intricate target...

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kikogarcia hat sein Profilbild aktualisiert
2024-07-04 08:22:03 - Übersetzen -

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