A major approach in the field of mammalian cell biology is the manipulation of the expression of the genes of interest in selected cell lines, to reveal one or several of the gene's function(s) using transient/stable overexpression or knockdown of the gene of interest. The simplest method for RNA interference is the cytosolic delivery of siRNA oligonucleotides, this technique is limited to cells capable of transfection and is primarily utilized during transient in vitro studies. The introduction of shRNA into mammalian cells through infection with viral vectors allows for stable integration of shRNA and long-term knockdown of the targeted gene. Creative Biolabs offers different approaches to knock down the membrane protein of interest.
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